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Express Enzyme (1X)培養基Gibco 12605010

價格:

規格: 100ml 100ml*10

聯系方式:I47-825O-882O

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Gibco表達酶(1X)酚紅培養基產品基本信息

產品名稱: Gibco Express Enzyme (1X)培養基、Gibco Express Enzyme (1X)酚紅培養基
產品又名: Gibco Express Enzyme (1X)細胞培養基、Gibco Express Enzyme (1X)酚紅細胞培養基
英文名稱: Gibco TrypLE Express Enzyme (1X), phenol red
中文名稱: 錐蟲 表達酶(1X)酚紅
培養基類型: 哺乳細胞培養基
級別: For Mammalian Cell Culture
品牌: Gibco
產品目錄號: 12605010,12605-010
螯合劑: EDTA,乙二胺四乙酸
分類: 不含動物源
酶穩定性: 室溫穩定
經過測試: 體外生物測定
細胞類型: 哺乳動物
養殖類型: 貼壁細胞培養
形式: 液體
酚紅指示劑: 含酚紅
試劑類型: 胰蛋白酶替代物
有效期: 24月
濃縮: 1X
產品類型: 細胞培養分離試劑
環保特征: 節能、可持續包裝
產品規格: 100ml
運輸條件: 室溫
保存條件: 密封,2-8°C保存。
注意事項: 避免呼入和皮膚接觸。

Gibco Express Enzyme (1X)培養試劑圖片

TrypLE&trade; Express Enzyme (1X), phenol red

Gibco Express Enzyme (1X)細胞培養試劑產品描述:

用重組酶取代動物胰蛋白酶從塑料上解離粘附的哺乳動物細胞。 TrypLE™ Express 中的酶與 TrypLE™ Select 相同,但采用了經濟型小包裝。 可以冷凍儲存,在室溫下可穩定儲存六個月。 TrypLE™ Express 可用于多種細胞,無需改變實驗方案。 無需使用胰蛋白酶抑制劑進行滅活。 在 PBS-EDTA 緩沖液中以 1X 濃度提供,并包含酚紅。
 
用于研究/進一步的細胞培養物生產。

Gibco Express Enzyme (1X)培養試劑配方

Components Molecular Weight Concentration (mg/L) mM
Inorganic Salts
Potassium Chloride (KCl) 75 200 2.6666667
Potassium Phosphate monobasic (KH2PO4) 136 200 1.4705882
Sodium Chloride (NaCl) 58 8000 137.93103
Sodium Phosphate dibasic (Na2HPO4-7H2O) 268 2160 8.059702
Other Components
EDTA 416 457.6 1.1
Phenol Red 376.4 100 0.26567483
rProtease   confidential n/a


Gibco細胞培養基選擇指南

Transfection Reagent (細胞轉染試劑)
產品描述 規格 貨號
Lipofectamin 2000 (Lipo2000、Lipo 2000)
Each unit contains one vial of Lipofectamine 2000 Reagent. 
0.75 ml 11668-027
11668027
Lipofectamin 3000 (Lipo3000、lipo 3000)
Contains one vial of Lipofectamine 3000 Reagent and one vial of P3000TM Enhancer Reagent.
0.75 ml L3000-008
L3000008
Dulbecco’s Modified Eagle Media (DMEM培養基)
產品描述 規格 貨號
DMEM (1X), liquid (high glucose)
Contains no L-glutamine or sodium pyruvate
500 mL 11960-044
11960044
DMEM (1X), liquid (high glucose)
Contains L-glutamine, but no sodium pyruvate
500 mL 11965-092
11965092
DMEM (1X), liquid (high glucose) (DMEM高糖培養基,最常用)
Contains L-glutamine and sodium pyruvate
500 mL 11995-065
11995065
DMEM (1X), liquid (high glucose)
Contains 110mg/L sodium pyruvate, but no L-glutamine
500 mL 10313-021
10313021
DMEM (1X), liquid (high glucose)
Contains 25 mM HEPES buffer, but no sodium pyruvate or phenol red
500 mL 21063-029
21063029
DMEM (1X), liquid (high glucose)
Contains no L-glutamine, sodium pyruvate, or phenol red
500 mL 31053-028
31053028
DMEM (1X), liquid (low glucose)
Contains L-glutamine and sodium pyruvate.
500 mL 11885-084
11885084
DMEM (1X), liquid (high glucose)
Contains L-glutamine, and 25 mM HEPES, but no sodium pyruvate
500 mL 12430-054
12430054
DMEM/F12 (1X), liquid 1:1
Contains L-glutamine, 15 mM HEPES butter, but no phenol red
500 mL 11039-021
11039021
DMEM/F12 (1X), liquid 1:1
Contains L-glutamine, but no HEPES buffer
500 mL 11320-033
11320033
DMEM/F12 (1X), liquid 1:1
Contains L-glutamine and 15 mM HEPES buffer
500 mL 11330-032
11330032
RPMI Media (RPMI1640培養基)
產品描述 規格 貨號
RPMI 1640 (1X), liquid(最常用)
Contains L-glutamine
500 mL 11875-093
11875093
RPMI 1640 (1X), liquid
Contains L-glutamine, but no phenol red
500 mL 11835-030
11835030
RPMI 1640 (1X), liquid
Contains no L-glutamine
500 mL 21870-076
21870076
RPMI 1640 (1X), liquid
Contains L-glutamine and 25 mM HEPES buffer
500 mL 22400-089
22400089
Minimum Essential Media (MEM培養基)
產品描述 規格 貨號
MEM Earle’s (1X), liquid
Contains Earle’s salts and L-glutamine
500 mL 11095-080
11095080
MEM Earle’s (1X), liquid
Contains Earle’s salts and non-essential amino acids, but no L-glutamine
500 mL 10370-021
10370021
MEM Earle’s (1X), liquid
Contains Earle’s salts, but no L-glutamine
500 mL 11090-081
11090081
MEM α (1X), liquid
Contains L-glutamine, ribonucleosides, and deoxyribonuclosides
500 mL 12571-063
12571063
MEM α Medium (1X), liquid
Contains L-glutamine, but no ribonucleoside or deoxyribonucleosides
500 mL 12561-056
12561056
MEM α Medium (1X), liquid
Contains L-glutamine, ribonucleosides and deoxyribonucleosides,
but no phenol red
500 mL 41061-029
41061029
GlutaMAX™ Media (GlutaMAX系列培養基)
產品描述 規格 貨號
DMEM (1X), liquid (high glucose)
Contains 110 mg/L sodium pyruvate
500 mL 10569-010
10569010
DMEM (1X), liquid (high glucose)
Contains 25 mM HEPES buffer, but no sodium pyruvate
500 mL 10564-011
10564011
DMEM (1X), liquid (high glucose)
Contains no sodium pyruvate
500 mL 10566-016
10566016
DMEM (1X), liquid (low glucose)
Contains 110 mg/L sodium pyruvate
500 mL 10567-014
10567014
DMEM/F12 (1X), liquid 1:1
Contains no HEPES buffer
500 mL 10565-018
10565018
F12 Nutrient Mixture (1X), liquid 500 mL 31765-035
31765035
IMDM (1X), liquid
Contains 25 mM HEPES buffer and 3,024 mg/L sodium bicarbonate
500 mL 31980-030
31980-030
MEM α (1X), liquid (最常用)
Contains no ribonucleosides or deoxyribonucleosides
500 mL 32561-037
32561037
MEM α (1X), liquid
Contains ribonucleosides and deoxyribonucleosides
500 mL 32571-036
32571036
MEM Earle’s (1X), liquid
Contains Earle’s salts
500 mL 41090-036
41090036
MEM Earle’s (1X), liquid
Contains Earle’s salts and 25 mM HEPES buffer
500 mL 42360-032
42360032
OptiMEM® I Reduced Serum Medium (1X), liquid 500 mL 51985-034
51985034
RPMI 1640 (1X), liquid 500 mL 61870-036
61870036
RPMI 1640 (1x), liquid
Contains 25 mM HEPES buffer
500 mL 72400-047
72400047
其他常用基礎培養基
產品描述 規格 貨號
F12 Nutrient Mixture (1X), liquid
Contains L-glutamine
500 mL 11765-054
11765054
F12K Nutrient Mixture, Kaighn’s Modification (1x), liquid
Contains L-glutamine
500 mL 21127-022
21127022
IMDM (1X), liquid
Contains L-glutamine, 25 mM HEPES buffer, 3,024 mg/L sodium bicarbonate, but no α-thioglycerol or 2-mercaptoethanol
  12440-053
12440053
Liebovitz’s L-15 Medium (1X), liquid (L15 Medium)
Contains L-glutamine
  11415-064
11415064
McCoy’s 5A Medium (modified) (1X), liquid
Contains L-glutamine
  16600-082
16600082
Medium 199 (1X), liquid
Contains Earle’s salts, L-glutamine, and 2,200 mg/L sodium bicarbonate
  11150-059
11150059
Reduced Serum Media 細胞減血清培養基
產品描述 規格 貨號
Advanced D-MEM (1X), liquid 500 mL 12491-015
12491015
Advanced D-MEM/F12 (1X), liquid 500 mL 12634-010
12634010
Advanced MEM (1X), liquid 500 mL 12492-013
12492013
Advanced RPMI 1640 (1X), liquid 500 mL 12633-012
12633012
Opti-MEM®-I Reduced Serum Medium (1X), liquid 500 mL 31985-070
31985070
Balanced Salt Solutions 細胞培養緩沖液
產品描述 規格 貨號
DPBS (1X), liquid
Contains calcium and magnesium
500 mL 14040-133
14040133
DPBS (1X), liquid
Contains no calcium or magnesium
500 mL 14190-144
14190144
DPBS (10X), liquid
Contains no calcium or magnesium
500 mL 14200-075
14200075
HBSS (1X), liquid
Contains calcium and magnesium, but no phenol red
500 mL 14025-092
14025092
HBSS (1X), liquid
Contains no calcium or magnesium
500 mL 14170-112
14170112
HBSS (1X), liquid
Contains no calcium chloride, magnesium chloride,
magnesium sulfate, or phenol red
500 mL 14175-095
14175-095
PBS 7.4 (1X), liquid 500 mL 10010-023
10010023
PBS 7.4 (10X), liquid 500 mL 70011-044
70011044
PBS 7.2 (1X), liquid 500 mL 20012-027
20012027
PBS 7.2 (10X), liquid 500 mL 70013-032
70013032
Insect Media 昆蟲培養基
產品描述 規格 貨號
Grace’s Insect Medium, Supplemented (1X), liquid
Contains 3,330 mg/L lactalbumin hydrolysate, 3,330 mg/L yeastolate, and L-glutamine, but no insect hemolymph
500 mL 11605-094
11605094
Schneider’s Drosophila Medium (1X), liquid
Contains L-glutamine
500 mL 11720-034
11720034
SF900-II SFM (1X), liquid
Contains L-Glutamine, Insect cell culture tested
1000 mL 10902-088
10902088
Reagents 細胞培養試劑
產品描述 規格 貨號
TrypLE™ Express Dissociation Enzyme with Phenol Red
Stable, non-animal origin, Trypsin-like enzyme. Equivalent to 0.25% Trypsin•EDTA
100 mL 12605-010
12605010
TrypLE™ Express Dissociation Enzyme without Phenol Red
Stable, non-animal origin, Trypsin-like enzyme. Equivalent to 0.25% Trypsin•EDTA
100 mL 12604-013
12604013
Trypsin-EDTA (0.25% Trypsin, EDTA•4Na) (1X), liquid(胰蛋白酶,最常用)
Contains 2.5 g/L of trypsin (1:250), 3.8 g/L of EDTA•4Na in Hanks’ Balanced Salt Solution without CaCl2, MgCl2•6H2O, and MgSO4•7H2O.
Contains phenol red
100 mL 25200-056
25200056
Trypsin-EDTA (0.05% Trypsin, EDTA•4Na) (1X), liquid
Contains 0.5 g/L trypsin (1:250), 0.2 g/L of EDTA•4Na in Hanks’ Balanced Salt Solution without CaCl2, MgCl2•6H2O, and MgSO4•7H2O.
Contains phenol red
100 mL 25300-054
25300054
HEPES Buffer Solution (1M)
Contains 238.3 g/L prepared in distilled water
100 mL 15630-080
15630080
L-glutamine-200 mM (100X), liquid 100 mL 25030-081
25030081
GlutaMAX™-I (100X), liquid 100 mL 35050-061
35050061
Antibiotic-Antimycotic (100X), liquid
10,000 units penicillin (base), 10,000 units streptomycin (base), and 25 μg amphotericin B/ml, utilizing penicillin G (sodium salt), streptomycin sulfate, and amphotericin B as Fungizone™ Antimycotic in 0.85% saline
100 mL 15240-062
15240062
Penicillin-Streptomycin, liquid (青鏈霉素雙抗)
10,000 Units penicillin (base) and 10,000 units streptomycin (base), utilizing penicillin G (sodium salt) and streptomycin sulfate in 0.85% saline
100 mL 15140-122
15140122
MEM Non-Essential Amino Acids Solution 10 mM (100X), liquid 100 mL 11140-050
11140050
Distilled Water(無菌水)
Membrane filtered and endotoxin screened
500 mL 15230-162
15230162

Gibco表達酶(1X)酚紅培養基產品說明書pdf版和相關資料下載

Gibco表達酶(1X)酚紅培養基產品應用舉例

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