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Advanced DMEM/F-12培養基Gibco 12634010

價格:

規格: 500ml 500ml*10

聯系方式:I47-825O-882O

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Gibco Advanced DMEM/F-12培養基產品基本信息

產品名稱: Gibco Advanced DMEM/F-12培養基、Gibco Advanced DMEM/F-12高糖培養基
產品又名: Gibco Advanced DMEM/F-12細胞培養基、Gibco Advanced DMEM/F-12細胞培養基
英文名稱: Gibco Advanced DMEM/F-12
培養基類型: 哺乳細胞培養基
級別: For Mammalian Cell Culture
品牌: Gibco
產品目錄號: 12634010,12634-010
葡萄糖: 高糖
谷氨酰胺: 不含L-谷氨酰胺
酚紅: 含酚紅
丙酮酸鈉: 含丙酮酸鈉
HEPES 不含HEPES
環保特征: 可循環使用包裝
產品規格: 500ml
血清添加: 使用時,正常濃度添加血清。
運輸條件: 室溫
產品外觀: 紅色液體
顏色與澄清度: 紅色透明至微不透明液體。
保存條件: 密封,2-8°C保存。
注意事項: 避免呼入和皮膚接觸。
Gibco: 新鮮酵母浸出液
熱滅活馬血清

Gibco Advanced DMEM/F-12培養基圖片

Advanced DMEM/F-12

Gibco Advanced DMEM/F-12細胞培養基產品描述:

Advanced DMEM/F-12(Dulbecco改良的Eagle培養基/Ham的F-12)是一種廣泛使用的基礎培養基,可用于哺乳動物細胞的培養。與傳統的DMEM/F-12相比,血清補充劑可以減少50-90%,而生長速度和形態沒有變化。在晚期DMEM/F-12中成功培養的細胞無適應性,包括MRC-5、SP2、Vero、WI-38和Jurkat。


Gibco Advanced DMEM/F-12培養基使用方法

高級DMEM/F-12與其他培養基不同,因為添加了以下成分以減少血清:乙醇胺、谷胱甘肽、抗壞血酸、胰島素、轉鐵蛋白、AlbuMAX®II細胞培養用富含脂質的牛血清白蛋白,以及微量元素亞硒酸鈉、偏釩酸銨、硫酸銅,氯化錳。晚期DMEM/F-12需要補充1-5%胎牛血清和4 mM L-谷氨酰胺或谷氨酰胺™ 補充。許多細胞系不需要適應這種介質。必須為每個細胞系優化FBS濃度,以獲得最大的血清還原率。先進的DMEM/F-12使用碳酸氫鈉緩沖系統(3.7 g/L),因此需要5–10%的二氧化碳環境來維持生理pH值。 

cGMP生產與質量體系

為了供應鏈的連續性,我們在紐約州格蘭德島和英國蘇格蘭的兩個獨立工廠生產先進的DMEM/F-12。這兩個工廠都符合cGMP生產要求,通過了ISO 13485認證,并在FDA注冊為醫療器械制造商。

Gibco Advanced DMEM/F-12培養基配方

Components Molecular Weight Concentration (mg/L) mM
Amino Acids
Glycine 75 18.75 0.25
L-Alanine 89 4.45 0.049999997
L-Arginine hydrochloride 211 147.5 0.69905216
L-Asparagine-H2O 150 7.5 0.05
L-Aspartic acid 133 6.65 0.05
L-Cysteine hydrochloride-H2O 176 17.56 0.09977272
L-Cystine 2HCl 313 31.29 0.09996805
L-Glutamic Acid 147 7.35 0.05
L-Histidine hydrochloride-H2O 210 31.48 0.14990476
L-Isoleucine 131 54.47 0.41580153
L-Leucine 131 59.05 0.45076334
L-Lysine hydrochloride 183 91.25 0.4986339
L-Methionine 149 17.24 0.11570469
L-Phenylalanine 165 35.48 0.2150303
L-Proline 115 17.25 0.15
L-Serine 105 26.25 0.25
L-Threonine 119 53.45 0.44915968
L-Tryptophan 204 9.02 0.04421569
L-Tyrosine disodium salt dihydrate 261 55.79 0.21375479
L-Valine 117 52.85 0.4517094
Vitamins
Ascorbic Acid phosphate 289.54 2.5 0.008634386
Biotin 244 0.0035 1.43E-05
Choline chloride 140 8.98 0.06414285
D-Calcium pantothenate 477 2.24 0.004696017
Folic Acid 441 2.65 0.006009071
Niacinamide 122 2.02 0.016557377
Pyridoxine hydrochloride 206 2 0.009708738
Riboflavin 376 0.219 5.82E-04
Thiamine hydrochloride 337 2.17 0.006439169
Vitamin B12 1355 0.68 5.02E-04
i-Inositol 180 12.6 0.07
Inorganic Salts
Calcium Chloride (CaCl2) (anhyd.) 111 116.6 1.0504504
Cupric sulfate (CuSO4-5H2O) 250 0.0013 5.20E-06
Ferric Nitrate (Fe(NO3)3"9H2O) 404 0.05 1.24E-04
Ferric sulfate (FeSO4-7H2O) 278 0.417 0.0015
Magnesium Chloride (anhydrous) 95 28.64 0.30147368
Magnesium Sulfate (MgSO4) (anhyd.) 120 48.84 0.407
Potassium Chloride (KCl) 75 311.8 4.1573334
Sodium Bicarbonate (NaHCO3) 84 2438 29.02381
Sodium Chloride (NaCl) 58 6995.5 120.61207
Sodium Phosphate dibasic (Na2HPO4) anhydrous 142 71.02 0.50014085
Sodium Phosphate monobasic (NaH2PO4-H2O) 138 62.5 0.45289856
Zinc sulfate (ZnSO4-7H2O) 288 0.864 0.003
Proteins
AlbuMAX® II   400 Infinity
Human Transferrin (Holo)   7.5 Infinity
Insulin Recombinant Full Chain   10 Infinity
Reducing Agents
Glutathione, monosodium 307 1 0.003257329
Trace Elements
Ammonium Metavanadate 116.98 3.00E-04 2.56E-06
Manganous Chloride 198 5.00E-05 2.53E-07
Sodium Selenite 173 0.005 2.89E-05
Other Components
D-Glucose (Dextrose) 180 3151 17.505556
Ethanolamine 97.54 1.9 0.019479187
Hypoxanthine Na 159 2.39 0.015031448
Linoleic Acid 280 0.042 1.50E-04
Lipoic Acid 206 0.105 5.10E-04
Phenol Red 376.4 8.1 0.021519661
Putrescine 2HCl 161 0.081 5.03E-04
Sodium Pyruvate 110 110 1
Thymidine 242 0.365 0.001508265


Gibco細胞培養基選擇指南

Transfection Reagent (細胞轉染試劑)
產品描述 規格 貨號
Lipofectamin 2000 (Lipo2000、Lipo 2000)
Each unit contains one vial of Lipofectamine 2000 Reagent. 
0.75 ml 11668-027
11668027
Lipofectamin 3000 (Lipo3000、lipo 3000)
Contains one vial of Lipofectamine 3000 Reagent and one vial of P3000TM Enhancer Reagent.
0.75 ml L3000-008
L3000008
Dulbecco’s Modified Eagle Media (DMEM培養基)
產品描述 規格 貨號
DMEM (1X), liquid (high glucose)
Contains no L-glutamine or sodium pyruvate
500 mL 11960-044
11960044
DMEM (1X), liquid (high glucose)
Contains L-glutamine, but no sodium pyruvate
500 mL 11965-092
11965092
DMEM (1X), liquid (high glucose) (DMEM高糖培養基,最常用)
Contains L-glutamine and sodium pyruvate
500 mL 11995-065
11995065
DMEM (1X), liquid (high glucose)
Contains 110mg/L sodium pyruvate, but no L-glutamine
500 mL 10313-021
10313021
DMEM (1X), liquid (high glucose)
Contains 25 mM HEPES buffer, but no sodium pyruvate or phenol red
500 mL 21063-029
21063029
DMEM (1X), liquid (high glucose)
Contains no L-glutamine, sodium pyruvate, or phenol red
500 mL 31053-028
31053028
DMEM (1X), liquid (low glucose)
Contains L-glutamine and sodium pyruvate.
500 mL 11885-084
11885084
DMEM (1X), liquid (high glucose)
Contains L-glutamine, and 25 mM HEPES, but no sodium pyruvate
500 mL 12430-054
12430054
DMEM/F12 (1X), liquid 1:1
Contains L-glutamine, 15 mM HEPES butter, but no phenol red
500 mL 11039-021
11039021
DMEM/F12 (1X), liquid 1:1
Contains L-glutamine, but no HEPES buffer
500 mL 11320-033
11320033
DMEM/F12 (1X), liquid 1:1
Contains L-glutamine and 15 mM HEPES buffer
500 mL 11330-032
11330032
RPMI Media (RPMI1640培養基)
產品描述 規格 貨號
RPMI 1640 (1X), liquid(最常用)
Contains L-glutamine
500 mL 11875-093
11875093
RPMI 1640 (1X), liquid
Contains L-glutamine, but no phenol red
500 mL 11835-030
11835030
RPMI 1640 (1X), liquid
Contains no L-glutamine
500 mL 21870-076
21870076
RPMI 1640 (1X), liquid
Contains L-glutamine and 25 mM HEPES buffer
500 mL 22400-089
22400089
Minimum Essential Media (MEM培養基)
產品描述 規格 貨號
MEM Earle’s (1X), liquid
Contains Earle’s salts and L-glutamine
500 mL 11095-080
11095080
MEM Earle’s (1X), liquid
Contains Earle’s salts and non-essential amino acids, but no L-glutamine
500 mL 10370-021
10370021
MEM Earle’s (1X), liquid
Contains Earle’s salts, but no L-glutamine
500 mL 11090-081
11090081
MEM α (1X), liquid
Contains L-glutamine, ribonucleosides, and deoxyribonuclosides
500 mL 12571-063
12571063
MEM α Medium (1X), liquid
Contains L-glutamine, but no ribonucleoside or deoxyribonucleosides
500 mL 12561-056
12561056
MEM α Medium (1X), liquid
Contains L-glutamine, ribonucleosides and deoxyribonucleosides,
but no phenol red
500 mL 41061-029
41061029
GlutaMAX™ Media (GlutaMAX系列培養基)
產品描述 規格 貨號
DMEM (1X), liquid (high glucose)
Contains 110 mg/L sodium pyruvate
500 mL 10569-010
10569010
DMEM (1X), liquid (high glucose)
Contains 25 mM HEPES buffer, but no sodium pyruvate
500 mL 10564-011
10564011
DMEM (1X), liquid (high glucose)
Contains no sodium pyruvate
500 mL 10566-016
10566016
DMEM (1X), liquid (low glucose)
Contains 110 mg/L sodium pyruvate
500 mL 10567-014
10567014
DMEM/F12 (1X), liquid 1:1
Contains no HEPES buffer
500 mL 10565-018
10565018
F12 Nutrient Mixture (1X), liquid 500 mL 31765-035
31765035
IMDM (1X), liquid
Contains 25 mM HEPES buffer and 3,024 mg/L sodium bicarbonate
500 mL 31980-030
31980-030
MEM α (1X), liquid (最常用)
Contains no ribonucleosides or deoxyribonucleosides
500 mL 32561-037
32561037
MEM α (1X), liquid
Contains ribonucleosides and deoxyribonucleosides
500 mL 32571-036
32571036
MEM Earle’s (1X), liquid
Contains Earle’s salts
500 mL 41090-036
41090036
MEM Earle’s (1X), liquid
Contains Earle’s salts and 25 mM HEPES buffer
500 mL 42360-032
42360032
OptiMEM® I Reduced Serum Medium (1X), liquid 500 mL 51985-034
51985034
RPMI 1640 (1X), liquid 500 mL 61870-036
61870036
RPMI 1640 (1x), liquid
Contains 25 mM HEPES buffer
500 mL 72400-047
72400047
其他常用基礎培養基
產品描述 規格 貨號
F12 Nutrient Mixture (1X), liquid
Contains L-glutamine
500 mL 11765-054
11765054
F12K Nutrient Mixture, Kaighn’s Modification (1x), liquid
Contains L-glutamine
500 mL 21127-022
21127022
IMDM (1X), liquid
Contains L-glutamine, 25 mM HEPES buffer, 3,024 mg/L sodium bicarbonate, but no α-thioglycerol or 2-mercaptoethanol
  12440-053
12440053
Liebovitz’s L-15 Medium (1X), liquid (L15 Medium)
Contains L-glutamine
  11415-064
11415064
McCoy’s 5A Medium (modified) (1X), liquid
Contains L-glutamine
  16600-082
16600082
Medium 199 (1X), liquid
Contains Earle’s salts, L-glutamine, and 2,200 mg/L sodium bicarbonate
  11150-059
11150059
Reduced Serum Media 細胞減血清培養基
產品描述 規格 貨號
Advanced D-MEM (1X), liquid 500 mL 12491-015
12491015
Advanced D-MEM/F12 (1X), liquid 500 mL 12634-010
12634010
Advanced MEM (1X), liquid 500 mL 12492-013
12492013
Advanced RPMI 1640 (1X), liquid 500 mL 12633-012
12633012
Opti-MEM®-I Reduced Serum Medium (1X), liquid 500 mL 31985-070
31985070
Balanced Salt Solutions 細胞培養緩沖液
產品描述 規格 貨號
DPBS (1X), liquid
Contains calcium and magnesium
500 mL 14040-133
14040133
DPBS (1X), liquid
Contains no calcium or magnesium
500 mL 14190-144
14190144
DPBS (10X), liquid
Contains no calcium or magnesium
500 mL 14200-075
14200075
HBSS (1X), liquid
Contains calcium and magnesium, but no phenol red
500 mL 14025-092
14025092
HBSS (1X), liquid
Contains no calcium or magnesium
500 mL 14170-112
14170112
HBSS (1X), liquid
Contains no calcium chloride, magnesium chloride,
magnesium sulfate, or phenol red
500 mL 14175-095
14175-095
PBS 7.4 (1X), liquid 500 mL 10010-023
10010023
PBS 7.4 (10X), liquid 500 mL 70011-044
70011044
PBS 7.2 (1X), liquid 500 mL 20012-027
20012027
PBS 7.2 (10X), liquid 500 mL 70013-032
70013032
Insect Media 昆蟲培養基
產品描述 規格 貨號
Grace’s Insect Medium, Supplemented (1X), liquid
Contains 3,330 mg/L lactalbumin hydrolysate, 3,330 mg/L yeastolate, and L-glutamine, but no insect hemolymph
500 mL 11605-094
11605094
Schneider’s Drosophila Medium (1X), liquid
Contains L-glutamine
500 mL 11720-034
11720034
SF900-II SFM (1X), liquid
Contains L-Glutamine, Insect cell culture tested
1000 mL 10902-088
10902088
Reagents 細胞培養試劑
產品描述 規格 貨號
TrypLE™ Express Dissociation Enzyme with Phenol Red
Stable, non-animal origin, Trypsin-like enzyme. Equivalent to 0.25% Trypsin•EDTA
100 mL 12605-010
12605010
TrypLE™ Express Dissociation Enzyme without Phenol Red
Stable, non-animal origin, Trypsin-like enzyme. Equivalent to 0.25% Trypsin•EDTA
100 mL 12604-013
12604013
Trypsin-EDTA (0.25% Trypsin, EDTA•4Na) (1X), liquid(胰蛋白酶,最常用)
Contains 2.5 g/L of trypsin (1:250), 3.8 g/L of EDTA•4Na in Hanks’ Balanced Salt Solution without CaCl2, MgCl2•6H2O, and MgSO4•7H2O.
Contains phenol red
100 mL 25200-056
25200056
Trypsin-EDTA (0.05% Trypsin, EDTA•4Na) (1X), liquid
Contains 0.5 g/L trypsin (1:250), 0.2 g/L of EDTA•4Na in Hanks’ Balanced Salt Solution without CaCl2, MgCl2•6H2O, and MgSO4•7H2O.
Contains phenol red
100 mL 25300-054
25300054
HEPES Buffer Solution (1M)
Contains 238.3 g/L prepared in distilled water
100 mL 15630-080
15630080
L-glutamine-200 mM (100X), liquid 100 mL 25030-081
25030081
GlutaMAX™-I (100X), liquid 100 mL 35050-061
35050061
Antibiotic-Antimycotic (100X), liquid
10,000 units penicillin (base), 10,000 units streptomycin (base), and 25 μg amphotericin B/ml, utilizing penicillin G (sodium salt), streptomycin sulfate, and amphotericin B as Fungizone™ Antimycotic in 0.85% saline
100 mL 15240-062
15240062
Penicillin-Streptomycin, liquid (青鏈霉素雙抗)
10,000 Units penicillin (base) and 10,000 units streptomycin (base), utilizing penicillin G (sodium salt) and streptomycin sulfate in 0.85% saline
100 mL 15140-122
15140122
MEM Non-Essential Amino Acids Solution 10 mM (100X), liquid 100 mL 11140-050
11140050
Distilled Water(無菌水)
Membrane filtered and endotoxin screened
500 mL 15230-162
15230162

Gibco Advanced DMEM/F-12培養基產品說明書pdf版和相關資料下載

Gibco Advanced DMEM/F-12培養基產品應用舉例

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