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RPMI 1640培養基Gibco 21870076

價格:

規格: 500ml 500ml*10

聯系方式:I47-825O-882O

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Gibco RPMI 1640無谷氨酰胺培養基產品基本信息

產品名稱: Gibco RPMI 1640培養基、Gibco RPMI 1640無谷氨酰胺培養基
產品又名: Gibco RPMI 1640細胞培養基、Gibco RPMI 1640無谷氨酰胺細胞培養基
英文名稱: Gibco RPMI 1640 Medium, no glutamine
培養基類型: 哺乳細胞培養基
級別: For Mammalian Cell Culture
品牌: Gibco
產品目錄號: 21870076、21870-076
谷氨酰胺: 不含L-谷氨酰胺
酚紅: 含酚紅
丙酮酸鈉: 不含丙酮酸鈉
HEPES 不含HEPES
環保特征: 可循環使用包裝
產品規格: 500ml
血清添加: 使用時,正常濃度添加血清。
運輸條件: 室溫
產品外觀: 紅色液體
顏色與澄清度: 紅色透明至微不透明液體。
保存條件: 密封,2-8°C保存。
注意事項: 避免呼入和皮膚接觸。
Gibco: 新鮮酵母浸出液
熱滅活馬血清

Gibco RPMI 1640培養基圖片

RPMI 1640 Medium, no glutamine

Gibco RPMI 1640細胞培養基產品描述:

RPMI1640培養基最初是用來培養懸浮和單層的人白血病細胞的。羅斯韋爾公園紀念研究所(RPMI)1640培養基已被發現適用于多種哺乳動物細胞,包括HeLa、Jurkat、MCF-7、PC12、PBMC、星形膠質細胞和癌。我們為一系列細胞培養應用提供多種RPMI 1640培養基修飾。使用介質選擇器工具找到正確的公式。

Gibco RPMI 1640培養基使用方法

RPMI 1640培養基與其他培養基不同,因為它含有還原劑谷胱甘肽和高濃度的維生素。RPMI 1640培養基含有生物素、維生素B12和PABA,在Eagle's Minimal Essential培養基或Dulbecco's Modified Eagle培養基中未發現。此外,維生素肌醇和膽堿的濃度非常高。RPMI 1640培養基不含蛋白質、脂類或生長因子。因此,RPMI 1640培養基需要補充,通常添加10%的胎牛血清(FBS)。RPMI 1640培養基使用碳酸氫鈉緩沖系統(2.0 g/L),因此需要5–10%的二氧化碳環境來維持生理pH值。

產品預期用途

用于體外診斷。注意:不可用于人類或動物治療。非預期用途可能違反當地法律。在生產過程中使用Gibco®RPMI 1640的客戶,如果已向FDA提交申請,可要求我們提供授權書,以參考我們的II型藥物主文件(DMF)。

Gibco RPMI 1640培養基生產與質量體系

RPMI 1640介質是在位于紐約格蘭德島的cGMP兼容工廠生產的。該設施在FDA注冊為醫療器械制造商,并通過ISO 13485標準認證。為了供應鏈的連續性,我們提供了一個相同的RPMI 1640產品在我們的蘇格蘭工廠(31870-074)。該工廠在FDA注冊為醫療器械制造商,并通過ISO 13485標準認證。

Gibco RPMI 1640培養基配方

Components Molecular Weight Concentration (mg/L) mM
Amino Acids
Glycine 75 10 0.13333334
L-Arginine 174 200 1.1494253
L-Asparagine 132 50 0.37878788
L-Aspartic acid 133 20 0.15037593
L-Cystine 2HCl 313 65 0.20766774
L-Glutamic Acid 147 20 0.13605443
L-Histidine 155 15 0.09677419
L-Hydroxyproline 131 20 0.15267175
L-Isoleucine 131 50 0.3816794
L-Leucine 131 50 0.3816794
L-Lysine hydrochloride 146 40 0.2739726
L-Methionine 149 15 0.10067114
L-Phenylalanine 165 15 0.09090909
L-Proline 115 20 0.17391305
L-Serine 105 30 0.2857143
L-Threonine 119 20 0.16806723
L-Tryptophan 204 5 0.024509804
L-Tyrosine disodium salt 225 29 0.12888889
L-Valine 117 20 0.17094018
Vitamins
Biotin 244 0.2 8.20E-04
Choline chloride 140 3 0.021428572
D-Calcium pantothenate 477 0.25 5.24E-04
Folic Acid 441 1 0.002267574
Niacinamide 122 1 0.008196721
Para-Aminobenzoic Acid 137 1 0.00729927
Pyridoxine hydrochloride 206 1 0.004854369
Riboflavin 376 0.2 5.32E-04
Thiamine hydrochloride 337 1 0.002967359
Vitamin B12 1355 0.005 3.69E-06
i-Inositol 180 35 0.19444445
Inorganic Salts
Calcium nitrate (Ca(NO3)2 4H2O) 236 100 0.42372882
Magnesium Sulfate (MgSO4-7H2O) 246 100 0.40650406
Potassium Chloride (KCl) 75 400 5.3333335
Sodium Bicarbonate (NaHCO3) 84 2000 23.809525
Sodium Chloride (NaCl) 58 6000 103.44827
Sodium Phosphate dibasic (Na2HPO4-7H2O) 268 1512 5.641791
Other Components
D-Glucose (Dextrose) 180 2000 11.111111
Glutathione (reduced) 307 1 0.003257329
Phenol Red 376.4 5 0.013283741


Gibco細胞培養基選擇指南

Transfection Reagent (細胞轉染試劑)
產品描述 規格 貨號
Lipofectamin 2000 (Lipo2000、Lipo 2000)
Each unit contains one vial of Lipofectamine 2000 Reagent. 
0.75 ml 11668-027
11668027
Lipofectamin 3000 (Lipo3000、lipo 3000)
Contains one vial of Lipofectamine 3000 Reagent and one vial of P3000TM Enhancer Reagent.
0.75 ml L3000-008
L3000008
Dulbecco’s Modified Eagle Media (DMEM培養基)
產品描述 規格 貨號
DMEM (1X), liquid (high glucose)
Contains no L-glutamine or sodium pyruvate
500 mL 11960-044
11960044
DMEM (1X), liquid (high glucose)
Contains L-glutamine, but no sodium pyruvate
500 mL 11965-092
11965092
DMEM (1X), liquid (high glucose) (DMEM高糖培養基,最常用)
Contains L-glutamine and sodium pyruvate
500 mL 11995-065
11995065
DMEM (1X), liquid (high glucose)
Contains 110mg/L sodium pyruvate, but no L-glutamine
500 mL 10313-021
10313021
DMEM (1X), liquid (high glucose)
Contains 25 mM HEPES buffer, but no sodium pyruvate or phenol red
500 mL 21063-029
21063029
DMEM (1X), liquid (high glucose)
Contains no L-glutamine, sodium pyruvate, or phenol red
500 mL 31053-028
31053028
DMEM (1X), liquid (low glucose)
Contains L-glutamine and sodium pyruvate.
500 mL 11885-084
11885084
DMEM (1X), liquid (high glucose)
Contains L-glutamine, and 25 mM HEPES, but no sodium pyruvate
500 mL 12430-054
12430054
DMEM/F12 (1X), liquid 1:1
Contains L-glutamine, 15 mM HEPES butter, but no phenol red
500 mL 11039-021
11039021
DMEM/F12 (1X), liquid 1:1
Contains L-glutamine, but no HEPES buffer
500 mL 11320-033
11320033
DMEM/F12 (1X), liquid 1:1
Contains L-glutamine and 15 mM HEPES buffer
500 mL 11330-032
11330032
RPMI Media (RPMI1640培養基)
產品描述 規格 貨號
RPMI 1640 (1X), liquid(最常用)
Contains L-glutamine
500 mL 11875-093
11875093
RPMI 1640 (1X), liquid
Contains L-glutamine, but no phenol red
500 mL 11835-030
11835030
RPMI 1640 (1X), liquid
Contains no L-glutamine
500 mL 21870-076
21870076
RPMI 1640 (1X), liquid
Contains L-glutamine and 25 mM HEPES buffer
500 mL 22400-089
22400089
Minimum Essential Media (MEM培養基)
產品描述 規格 貨號
MEM Earle’s (1X), liquid
Contains Earle’s salts and L-glutamine
500 mL 11095-080
11095080
MEM Earle’s (1X), liquid
Contains Earle’s salts and non-essential amino acids, but no L-glutamine
500 mL 10370-021
10370021
MEM Earle’s (1X), liquid
Contains Earle’s salts, but no L-glutamine
500 mL 11090-081
11090081
MEM α (1X), liquid
Contains L-glutamine, ribonucleosides, and deoxyribonuclosides
500 mL 12571-063
12571063
MEM α Medium (1X), liquid
Contains L-glutamine, but no ribonucleoside or deoxyribonucleosides
500 mL 12561-056
12561056
MEM α Medium (1X), liquid
Contains L-glutamine, ribonucleosides and deoxyribonucleosides,
but no phenol red
500 mL 41061-029
41061029
GlutaMAX™ Media (GlutaMAX系列培養基)
產品描述 規格 貨號
DMEM (1X), liquid (high glucose)
Contains 110 mg/L sodium pyruvate
500 mL 10569-010
10569010
DMEM (1X), liquid (high glucose)
Contains 25 mM HEPES buffer, but no sodium pyruvate
500 mL 10564-011
10564011
DMEM (1X), liquid (high glucose)
Contains no sodium pyruvate
500 mL 10566-016
10566016
DMEM (1X), liquid (low glucose)
Contains 110 mg/L sodium pyruvate
500 mL 10567-014
10567014
DMEM/F12 (1X), liquid 1:1
Contains no HEPES buffer
500 mL 10565-018
10565018
F12 Nutrient Mixture (1X), liquid 500 mL 31765-035
31765035
IMDM (1X), liquid
Contains 25 mM HEPES buffer and 3,024 mg/L sodium bicarbonate
500 mL 31980-030
31980-030
MEM α (1X), liquid (最常用)
Contains no ribonucleosides or deoxyribonucleosides
500 mL 32561-037
32561037
MEM α (1X), liquid
Contains ribonucleosides and deoxyribonucleosides
500 mL 32571-036
32571036
MEM Earle’s (1X), liquid
Contains Earle’s salts
500 mL 41090-036
41090036
MEM Earle’s (1X), liquid
Contains Earle’s salts and 25 mM HEPES buffer
500 mL 42360-032
42360032
OptiMEM® I Reduced Serum Medium (1X), liquid 500 mL 51985-034
51985034
RPMI 1640 (1X), liquid 500 mL 61870-036
61870036
RPMI 1640 (1x), liquid
Contains 25 mM HEPES buffer
500 mL 72400-047
72400047
其他常用基礎培養基
產品描述 規格 貨號
F12 Nutrient Mixture (1X), liquid
Contains L-glutamine
500 mL 11765-054
11765054
F12K Nutrient Mixture, Kaighn’s Modification (1x), liquid
Contains L-glutamine
500 mL 21127-022
21127022
IMDM (1X), liquid
Contains L-glutamine, 25 mM HEPES buffer, 3,024 mg/L sodium bicarbonate, but no α-thioglycerol or 2-mercaptoethanol
  12440-053
12440053
Liebovitz’s L-15 Medium (1X), liquid (L15 Medium)
Contains L-glutamine
  11415-064
11415064
McCoy’s 5A Medium (modified) (1X), liquid
Contains L-glutamine
  16600-082
16600082
Medium 199 (1X), liquid
Contains Earle’s salts, L-glutamine, and 2,200 mg/L sodium bicarbonate
  11150-059
11150059
Reduced Serum Media 細胞減血清培養基
產品描述 規格 貨號
Advanced D-MEM (1X), liquid 500 mL 12491-015
12491015
Advanced D-MEM/F12 (1X), liquid 500 mL 12634-010
12634010
Advanced MEM (1X), liquid 500 mL 12492-013
12492013
Advanced RPMI 1640 (1X), liquid 500 mL 12633-012
12633012
Opti-MEM®-I Reduced Serum Medium (1X), liquid 500 mL 31985-070
31985070
Balanced Salt Solutions 細胞培養緩沖液
產品描述 規格 貨號
DPBS (1X), liquid
Contains calcium and magnesium
500 mL 14040-133
14040133
DPBS (1X), liquid
Contains no calcium or magnesium
500 mL 14190-144
14190144
DPBS (10X), liquid
Contains no calcium or magnesium
500 mL 14200-075
14200075
HBSS (1X), liquid
Contains calcium and magnesium, but no phenol red
500 mL 14025-092
14025092
HBSS (1X), liquid
Contains no calcium or magnesium
500 mL 14170-112
14170112
HBSS (1X), liquid
Contains no calcium chloride, magnesium chloride,
magnesium sulfate, or phenol red
500 mL 14175-095
14175-095
PBS 7.4 (1X), liquid 500 mL 10010-023
10010023
PBS 7.4 (10X), liquid 500 mL 70011-044
70011044
PBS 7.2 (1X), liquid 500 mL 20012-027
20012027
PBS 7.2 (10X), liquid 500 mL 70013-032
70013032
Insect Media 昆蟲培養基
產品描述 規格 貨號
Grace’s Insect Medium, Supplemented (1X), liquid
Contains 3,330 mg/L lactalbumin hydrolysate, 3,330 mg/L yeastolate, and L-glutamine, but no insect hemolymph
500 mL 11605-094
11605094
Schneider’s Drosophila Medium (1X), liquid
Contains L-glutamine
500 mL 11720-034
11720034
SF900-II SFM (1X), liquid
Contains L-Glutamine, Insect cell culture tested
1000 mL 10902-088
10902088
Reagents 細胞培養試劑
產品描述 規格 貨號
TrypLE™ Express Dissociation Enzyme with Phenol Red
Stable, non-animal origin, Trypsin-like enzyme. Equivalent to 0.25% Trypsin•EDTA
100 mL 12605-010
12605010
TrypLE™ Express Dissociation Enzyme without Phenol Red
Stable, non-animal origin, Trypsin-like enzyme. Equivalent to 0.25% Trypsin•EDTA
100 mL 12604-013
12604013
Trypsin-EDTA (0.25% Trypsin, EDTA•4Na) (1X), liquid(胰蛋白酶,最常用)
Contains 2.5 g/L of trypsin (1:250), 3.8 g/L of EDTA•4Na in Hanks’ Balanced Salt Solution without CaCl2, MgCl2•6H2O, and MgSO4•7H2O.
Contains phenol red
100 mL 25200-056
25200056
Trypsin-EDTA (0.05% Trypsin, EDTA•4Na) (1X), liquid
Contains 0.5 g/L trypsin (1:250), 0.2 g/L of EDTA•4Na in Hanks’ Balanced Salt Solution without CaCl2, MgCl2•6H2O, and MgSO4•7H2O.
Contains phenol red
100 mL 25300-054
25300054
HEPES Buffer Solution (1M)
Contains 238.3 g/L prepared in distilled water
100 mL 15630-080
15630080
L-glutamine-200 mM (100X), liquid 100 mL 25030-081
25030081
GlutaMAX™-I (100X), liquid 100 mL 35050-061
35050061
Antibiotic-Antimycotic (100X), liquid
10,000 units penicillin (base), 10,000 units streptomycin (base), and 25 μg amphotericin B/ml, utilizing penicillin G (sodium salt), streptomycin sulfate, and amphotericin B as Fungizone™ Antimycotic in 0.85% saline
100 mL 15240-062
15240062
Penicillin-Streptomycin, liquid (青鏈霉素雙抗)
10,000 Units penicillin (base) and 10,000 units streptomycin (base), utilizing penicillin G (sodium salt) and streptomycin sulfate in 0.85% saline
100 mL 15140-122
15140122
MEM Non-Essential Amino Acids Solution 10 mM (100X), liquid 100 mL 11140-050
11140050
Distilled Water(無菌水)
Membrane filtered and endotoxin screened
500 mL 15230-162
15230162

Gibco RPMI 1640無谷氨酰胺培養基產品說明書pdf版和相關資料下載

Gibco RPMI 1640無谷氨酰胺培養基產品應用舉例

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