成人欧美一区二区三区黑人,无套中出丰满人妻无码,爱色精品视频一区二区

<small id="gc8ki"><wbr id="gc8ki"></wbr></small>
<div id="gc8ki"><s id="gc8ki"></s></div><div id="gc8ki"><button id="gc8ki"></button></div><div id="gc8ki"><wbr id="gc8ki"></wbr></div>
<small id="gc8ki"><wbr id="gc8ki"></wbr></small>
<div id="gc8ki"><wbr id="gc8ki"></wbr></div>
<small id="gc8ki"><wbr id="gc8ki"></wbr></small>
<div id="gc8ki"><wbr id="gc8ki"></wbr></div><small id="gc8ki"><wbr id="gc8ki"></wbr></small>
<small id="gc8ki"><wbr id="gc8ki"></wbr></small>

pBMTB-1

價格:2500元

聯系方式:I47-825O-882O

相關技術服務:質粒構建    基因合成    質粒大提

買家導航

載體基本信息

出品人: Lynch,M.D. and Gill,R.T.
載體名稱: pBMTB-1
質粒類型: 廣宿主載體;克隆載體;原核表達載體
高拷貝/低拷貝: --
克隆方法: 限制性內切酶,多克隆位點
啟動子: araBAD
載體大小: 5029 bp
5' 測序引物及序列: --
3' 測序引物及序列: --
載體標簽: --
載體抗性: 氨芐青霉素
克隆菌株: DH5α 等
宿主細胞(系): 多個菌屬
備注: --
產品目錄號: --
組成型/誘導型: 誘導型

載體質粒圖譜和多克隆位點信息

pBMTB-1載體圖譜



載體簡介

載體序列

LOCUS       DQ058724                5029 bp    DNA     circular SYN 08-MAY-2006
DEFINITION  Broad host range vector pBMTB-1, complete sequence.
ACCESSION   DQ058724
VERSION     DQ058724.1  GI:69048156
  AUTHORS   Lynch,M.D. and Gill,R.T.
  TITLE     Broad host range vectors for stable genomic library construction
   PUBMED   16496398
  JOURNAL   Submitted (12-MAY-2005) Chemical & Biological Engineering,
            University of Colorado, ECCH 111, Campus Box 424, Boulder, CO
            80309, USA
FEATURES             Location/Qualifiers
     source          1..5029
                     /organism="Broad host range vector pBMTB-1"
                     /mol_type="other DNA"
                     /db_xref="taxon:334956"
     regulatory      60..96
                     /regulatory_class="terminator"
                     /note="SoxR terminator"
     gene            complement(104..1123)
                     /gene="mob"
                     /note="mob gene from pBBR1MCS, GenBank Accession Number
                     U02374"
     CDS             complement(104..1123)
                     /gene="mob"
                     /note="required for mobilization"
                     /codon_start=1
                     /transl_table=11
                     /product="Mob"
                     /protein_id="AAY99697.1"
                     /db_xref="GI:69048157"
                     /translation="MAAYAIMRCKKLAKMGNVAASLKHAYRERETPNADASRTPENEH
                     WAASSTDEAMGRLRELLPEKRRKDAVLAVEYVMTASPEWWKSASQEQQAAFFEKAHKW
                     LADKYGADRIVTASIHRDETSPHMTAFVVPLTQDGRLSAKEFIGNKAQMTRDQTTFAA
                     AVADLGLQRGIEGSKARHTRIQAFYEALERPPVGHVTISPQAVEPRAYAPQGLAEKLG
                     ISKRVETPEAVADRLTKAVRQGYEPALQAAAGAREMRKKADQAQETARDLRERLKPVL
                     DALGPLNRDMQAKAAAIIKAVGEKLLTEQREVQRQKQAQRQQERGRAHFPEKCHLTSK
                     KPLLS"
     gene            2117..2779
                     /gene="rep"
                     /note="rep gene from pBBR1, GenBank Accession Number
                     X66730"
     CDS             2117..2779
                     /gene="rep"
                     /note="required for replication"
                     /codon_start=1
                     /transl_table=11
                     /product="Rep"
                     /protein_id="AAY99698.1"
                     /db_xref="GI:69048158"
                     /translation="MATQSREIGIQAKNKPGHWVQTERKAHEAWAGLIARKPTAAMLL
                     HHLVAQMGHQNAVVVSQKTLSKLIGRSLRTVQYAVKDLVAERWISVVKLNGPGTVSAY
                     VVNDRVAWGQPRDQLRLSVFSAAVVVDHDDQDESLLGHGDLRRIPTLYPGEQQLPTGP
                     GEEPPSQPGIPGMEPDLPALTETEEWERRGQQRLPMPDEPCFLDDGEPLEPPTRVTLP
                     RR"
     gene            2876..3736
                     /gene="bla"
                     /note="beta-lactam resistance"
     CDS             2876..3736
                     /gene="bla"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase"
                     /protein_id="AAY99699.1"
                     /db_xref="GI:69048159"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESLRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLATIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGSL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW"
     regulatory      3752..3783
                     /regulatory_class="terminator"
                     /note="TonB terminator"
     gene            complement(3811..4740)
                     /gene="araC"
     CDS             complement(3811..4740)
                     /gene="araC"
                     /note="regulator"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC"
                     /protein_id="AAY99700.1"
                     /db_xref="GI:69048160"
                     /translation="MQYGQLVSSLNGGSMKSMAEAQNDPLLPGYSFNAHLVAGLTPIE
                     ANGYLDFFIDRPLGMKGYILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRH
                     PEAREWYHQWVYFRPRAYWHEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQG
                     EGRYSELLAINLLEQLLLRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVA
                     QHVCLSPSRLSHLFRQQLGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQL
                     YFSRVFKKCTGASPSEFRAGCEEKVNDVAVKLS"
     regulatory      4748..5015
                     /regulatory_class="promoter"
                     /note="pBAD promoter"
ORIGIN      
        1 aacgaattca agcttgatat cattcaggac gagcctcaga ctccagcgta actggactga
       61 aaacaaacta aagcgccctt gtggcgcttt agttttgttc cgctcatgat aataatggtt
      121 tcttagacgt caggtggcac ttttcgggga aatgtgcgcg cccgcgttcc tgctggcgct
      181 gggcctgttt ctggcgctgg acttcccgct gttccgtcag cagcttttcg cccacggcct
      241 tgatgatcgc ggcggccttg gcctgcatat cccgattcaa cggccccagg gcgtccagaa
      301 cgggcttcag gcgctcccga aggtctcggg ccgtctcttg ggcttgatcg gccttcttgc
      361 gcatctcacg cgctcctgcg gcggcctgta gggcaggctc atacccctgc cgaaccgctt
      421 ttgtcagccg gtcggccacg gcttccggcg tctcaacgcg ctttgagatt cccagctttt
      481 cggccaatcc ctgcggtgca taggcgcgtg gctcgaccgc ttgcgggctg atggtgacgt
      541 ggcccactgg tggccgctcc agggcctcgt agaacgcctg aatgcgcgtg tgacgtgcct
      601 tgctgccctc gatgccccgt tgcagcccta gatcggccac agcggccgca aacgtggtct
      661 ggtcgcgggt catctgcgct ttgttgccga tgaactcctt ggccgacagc ctgccgtcct
      721 gcgtcagcgg caccacgaac gcggtcatgt gcgggctggt ttcgtcacgg tggatgctgg
      781 ccgtcacgat gcgatccgcc ccgtacttgt ccgccagcca cttgtgcgcc ttctcgaaga
      841 acgccgcctg ctgttcttgg ctggccgact tccaccattc cgggctggcc gtcatgacgt
      901 actcgaccgc caacacagcg tccttgcgcc gcttctctgg cagcaactcg cgcagtcggc
      961 ccatcgcttc atcggtgctg ctggccgccc agtgctcgtt ctctggcgtc ctgctggcgt
     1021 cagcgttggg cgtctcgcgc tcgcggtagg cgtgcttgag actggccgcc acgttgccca
     1081 ttttcgccag cttcttgcat cgcatgatcg cgtatgccgc catgcctgcc cctccctttt
     1141 ggtgtccaac cggctcgacg ggggcagcgc aaggcggtgc ctccggcggg ccactcaatg
     1201 cttgagtata ctcactagac tttgcttcgc aaagtcgtga ccgcctacgg cggctgcggc
     1261 gccctacggg cttgctctcc gggcttcgcc ctgcgcggtc gctgcgctcc cttgccagcc
     1321 cgtggatatg tggacgatgg ccgcgagcgg ccaccggctg gctcgcttcg ctcggcccgt
     1381 ggacaaccct gctggacaag ctgatggaca ggctgcgcct gcccacgagc ttgaccacag
     1441 ggattgccca ccggctaccc agccttcgac cacataccca ccggctccaa ctgcgcggcc
     1501 tgcggccttg ccccatcaat ttttttaatt ttctctgggg aaaagcctcc ggcctgcggc
     1561 ctgcgcgctt cgcttgccgg ttggacacca agtggaaggc gggtcaaggc tcgcgcagcg
     1621 accgcgcagc ggcttggcct tgacgcgcct ggaacgaccc aagcctatgc gagtgggggc
     1681 agtcgaaggc gaagcccgcc cgcctgcccc ccgagcctca cggcggcgag tgcgggggtt
     1741 ccaagggggc agcgccacct tgggcaaggc cgaaggccgc gcagtcgatc aacaagcccc
     1801 ggaggggcca ctttttgccg gagggggagc cgcgccgaag gcgtggggga accccgcagg
     1861 ggtgcccttc tttgggcacc aaagaactag atatagggcg aaatgcgaaa gacttaaaaa
     1921 tcaacaactt aaaaaagggg ggtacgcaac agctcattgc ggcacccccc gcaatagctc
     1981 attgcgtagg ttaaagaaaa tctgtaattg actgccactt ttacgcaacg cataattgtt
     2041 gtcgcgctgc cgaaaagttg cagctgattg cgcatggtgc cgcaaccgtg cggcacccta
     2101 ccgcatggag ataagcatgg ccacgcagtc cagagaaatc ggcattcaag ccaagaacaa
     2161 gcccggtcac tgggtgcaaa cggaacgcaa agcgcatgag gcgtgggccg ggcttattgc
     2221 gaggaaaccc acggcggcaa tgctgctgca tcacctcgtg gcgcagatgg gccaccagaa
     2281 cgccgtggtg gtcagccaga agacactttc caagctcatc ggacgttctt tgcggacggt
     2341 ccaatacgca gtcaaggact tggtggccga gcgctggatc tccgtcgtga agctcaacgg
     2401 ccccggcacc gtgtcggcct acgtggtcaa tgaccgcgtg gcgtggggcc agccccgcga
     2461 ccagttgcgc ctgtcggtgt tcagtgccgc cgtggtggtt gatcacgacg accaggacga
     2521 atcgctgttg gggcatggcg acctgcgccg catcccgacc ctgtatccgg gcgagcagca
     2581 actaccgacc ggccccggcg aggagccgcc cagccagccc ggcattccgg gcatggaacc
     2641 agacctgcca gccttgaccg aaacggagga atgggaacgg cgcgggcagc agcgcctgcc
     2701 gatgcccgat gagccgtgtt ttctggacga tggcgagccg ttggagccgc cgacacgggt
     2761 cacgctgccg cgccggtagt acgtaagagg ttccaacttt caccataatg aaataagatc
     2821 actaccgggc gtattttttg agttatcgag attttcagga gctaaggaag ctaaaatgag
     2881 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     2941 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     3001 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagtttac gccccgaaga
     3061 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     3121 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     3181 gtactcacca gtcacagaaa agcatctcac ggatggcatg acagtaagag aattatgcag
     3241 tgctgccata accatgagtg ataacactgc ggccaactta cttctggcaa cgatcggagg
     3301 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     3361 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     3421 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     3481 gcaacaatta atagactgga tggaggcgga taaagttgca ggatcacttc tgcgctcggc
     3541 cctcccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     3601 tatcattgca gcactggggc cagatggtaa gccctcccgc atcgtagtta tctacacgac
     3661 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     3721 gattaagcat tggtaatgag gatccccctc aagtcaaaag cctccggtcg gaggcttttg
     3781 actttctgct atggaggtca ggtatgattt ttatgacaac ttgacggcta catcattcac
     3841 tttttcttca caaccggcac ggaactcgct cgggctggcc ccggtgcatt ttttaaatac
     3901 ccgcgagaaa tagagttgat cgtcaaaacc aacattgcga ccgacggtgg cgataggcat
     3961 ccgggtggtg ctcaaaagca gcttcgcctg gctgatacgt tggtcctcgc gccagcttaa
     4021 gacgctaatc cctaactgct ggcggaaaag atgtgacaga cgcgacggcg acaagcaaac
     4081 atgctgtgcg acgctggcga tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg
     4141 acaagcctcg cgtacccgat tatccatcgg tggatggagc gactcgttaa tcgcttccat
     4201 gcgccgcagt aacaattgct caagcagatt tatcgccagc agctccgaat agcgcccttc
     4261 cccttgcccg gcgttaatga tttgcccaaa caggtcgctg aaatgcggct ggtgcgcttc
     4321 atccgggcga aagaaccccg tattggcaaa tattgacggc cagttaagcc attcatgcca
     4381 gtaggcgcgc ggacgaaagt aaacccactg gtgataccat tcgcgagcct ccggatgacg
     4441 accgtagtga tgaatctctc ctggcgggaa cagcaaaata tcacccggtc ggcaaacaaa
     4501 ttctcgtccc tgatttttca ccaccccctg accgcgaatg gtgagattga gaatataacc
     4561 tttcattccc agcggtcggt cgataaaaaa atcgagataa ccgttggcct caatcggcgt
     4621 taaacccgcc accagatggg cattaaacga gtatcccggc agcaggggat cattttgcgc
     4681 ttcagccata cttttcatac tcccgccatt cagagaagaa accaattgtc catattgcat
     4741 cagacattgc cgtcactgcg tcttttactg gctcttctcg ctaaccaaac cggtaacccc
     4801 gcttattaaa agcattctgt aacaaagcgg gaccaaagcc atgacaaaaa cgcgtaacaa
     4861 aagtgtctat aatcacggca gaaaagtcca cattgattat ttgcacggcg tcacactttg
     4921 ctatgccata gcatttttat ccataagatt agcggatcct acctgacgct ttttatcgca
     4981 actctctact gtttctccat acccgttttt ttgggctaga gaattcgtc
//

載體質粒圖譜pdf版和相關資料下載

載體應用舉例

分享到:

全部載體分類

相關載體

相關文章

相關問答

Copyright © 20012-2013 BIOFENG. 生物風 版權所有 Powered by Biofeng 滬ICP備2021037978號-1
歡迎您的訪問和咨詢....
成人欧美一区二区三区黑人,无套中出丰满人妻无码,爱色精品视频一区二区