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pPTRI

價格:4800元

聯系方式:I47-825O-882O

相關技術服務:質粒構建    基因合成    質粒大提

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pPTR I載體基本信息

出品公司: Takara
載體名稱: pPTRI
質粒類型: 曲霉表達質粒,大腸桿菌-曲霉穿梭質粒,曲霉表達載體
高拷貝/低拷貝: 高拷貝
克隆方法: 限制性內切酶,多克隆位點
啟動子: Lac
載體大小: 4782 bp
5’測序引物: M13-R: CAGGAAACAGCTATGAC
3’測序引物: M13-F: TGTAAAACGACGGCCAGT
載體抗性: 氨芐青霉素
克隆菌株: DH5 alpha
宿主細胞(系): 曲霉菌
產品目錄號: 3621
穩定性: 瞬表達
組成型/誘導型: 組成型
病毒/非病毒: 非病毒
相關載體: pPTRII
載體說明書: pPTRI載體說明書
備注: 本載體是曲霉表達載體,可以用于多種曲霉菌(Aspergillus species),例如A. oryzae, A . niger, A. terreus and A . fumigatus。pPTRI載體在曲霉菌中的篩選抗性是Pyrithiamine,中文名是抗硫胺素, 也叫吡啶硫銨。
本載體在構建質粒時,可以利用藍白斑篩選進行陽性插入克隆驗證。

pPTR I載體質粒圖譜和多克隆位點信息

pPTRI載體圖譜

pPTRI多克隆位點

pPTR I載體簡介

pPTR I是一種染色體整合的大腸桿菌曲霉菌穿梭載體。在曲霉菌細胞中,pPTR I不能自主復制,只有當整合到染色體上才能維持質粒穩定性。該載體具有氨芐西林抗性基因 Ampr作為大腸桿菌篩選抗性。

在曲霉中,篩選基因是抗硫胺素(Pyrithiamine) ,抗性基因為ptr A基因。本質粒具有多克隆位點,包含 Hind III,Pst I,Sma I和Kpn I。

在載體lac Z區域,任何DNA插入到這個區域 使用含有IPTG和X-Gal的平板可以很容易地進行藍白斑篩選陽性克隆載體。

pPTR I載體序列

LOCUS       pPTR I Vector                4782 bp DNA     circular SYN 23-JUL-2021
COMMENT     Created by Takara Bio Inc. http://www.altiliburda.com
COMMENT     Cat. No. 3621
FEATURES             Location/Qualifiers
     gene            complement(310..2317)
                     /label="ptrA"
                     /note="Pyrithiamine resistance gene useful as a dominant selectable marker in 
                     Aspergillus"
     CDS             2335..3195
                     /label="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
     rep_origin      3366..3954
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     promoter        4278..4308
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    4316..4332
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4340..4356
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             4352..4678
                     /label="lacZ-alpha"
     misc_feature    4369..4425
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(4426..4442)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
       61 cggcacctcg accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc
      121 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
      181 ttccaaactg gaacaacact caactctatc tcgggctatt cttttgattt ataagggatt
      241 ttgccgattt cggtctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
      301 tttaacaaaa tggggtgacg atgagccgct cttgcatctt tgtttgtatt atactgtctt
      361 tcttgttaca cataattatt ctagaatgcc ccaccgttac atacgggaca cagccattta
      421 catatgcatg tggattacga gctaacgagt tcattcaaat ctcagaacta tcacataatc
      481 atcattcccc tatcgtcaaa gaccgtaaga caaatccggt tcatgcactg aacccattcg
      541 ggtagtgagt catttactca gcacactcgc gctgacgctc gtcgaacacc ttcaatgcct
      601 cctcggcagc cttgacacca ctgagaacca tggcaccgaa ggtagggccc atgcggttaa
      661 agccatcaat ttcagacagc tccataccgc cgattatcaa gcccttagta acctcgcggg
      721 tgttcttgac gatggcatcc tcggccgagc tcatgtcgag accacgcatg ccacctagct
      781 tgtcgacgct gcccatggac accaagcgct tcgcacagaa ggcgccgaat ggcccatcgt
      841 gaccagtggt actgatgatg acaggagcgt tgatagtgtt ggggtccatg caggagtgat
      901 catcgtggtg aagggtgacc agcgtccagt tgacgacaac accagcaatc tgggggttgc
      961 cgttctcggt cggacgggtg atcaagtcct caacagcggt agcattgaag agcttgacat
     1021 tggggaagga gagaaccttc gacatgagtg tcgaggtaaa cagggaggcg tgcttgacga
     1081 caacgtagtt ggggtttgcg tcctcttcgt aaggaacacc cagctcgttc aggaagactt
     1141 ccgcgggacg gcgcatgacc atagcagaaa agagttggcc acccaaccag gcaccgccac
     1201 ctattgcacg ttagttccgg aaagctgagt gcaaggcaat ccatcatgga ctactgacca
     1261 ggagagacgc tggcctcgac gatagcaatc ttcaggtccg gacgagcctt ggccaagacg
     1321 tacgcagtgc tcagaccgca ggaaccagca ccaacaatga caacgtcact ttcagcgtac
     1381 ttgtccaggt cctcaaagta acgtctggtc atggcacgag agacctggct ttcgcggata
     1441 ggggcgaact tgaactcgtc ccacttgcca ccgaaatggt ccaacagctt ggtctgagaa
     1501 gctccctcaa cggggacggt ctcagaaacc acgaccttac ccttgaggcc ggtagcggcc
     1561 acagtgggtt cgtagatggc agctggagga gacatgtttc aagttgcaat gactatcatc
     1621 tgttagccat tccatcaaca ggaagaacga gagaaggcat gacccttttc gctggtatta
     1681 tccagatcaa gttttagccg tataatctca gaacgaaccc agtccatcga tgccatgtcc
     1741 ttctagacta ggatcctaga gtctagggcc cagcttaggg agggcatgtg aatgcatcga
     1801 tgactgggaa cgaacaccgg cccacgccaa agacgttacc taagatacct tgatcattgt
     1861 gagagtccag ccaaaagtat tccatgactt ccatcgtatg ccctctagag ggctaatcga
     1921 ggagtgtatt tacattgtcg gttggtttgg gaactataga agatggtcag ttattccaat
     1981 caccaaaggt ttatcgaagg gaggaagact tgttcagttt cgtccgagga cttttggaat
     2041 tcaaatctga gatagagaat tgtgtgggat gagaggaaaa ggaagaagat ggtggggttc
     2101 agaaggaggg gttgagttaa atagcatggg ttgagtcaac gtgataaggg cactataccg
     2161 tatagatcag cggcacccga ttctatccgt tccttttgct cctctttagc tttgaccggt
     2221 gagccggaca agaaacaagt gaaatcatcc tgacatcggc ggacgatctc ctagctttta
     2281 catttcgtta ccaatgggat cccgtaatca attgcccatt gaaaaaggaa gagtatgagt
     2341 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     2401 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     2461 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     2521 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     2581 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     2641 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     2701 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     2761 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     2821 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     2881 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     2941 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     3001 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     3061 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     3121 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     3181 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     3241 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     3301 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     3361 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     3421 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     3481 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     3541 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     3601 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     3661 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     3721 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     3781 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     3841 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     3901 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     3961 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     4021 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     4081 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     4141 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     4201 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     4261 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     4321 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gcttgcatgc
     4381 ctgcaggtcg actctagagg atccccgggt accgagctcg aattcactgg ccgtcgtttt
     4441 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
     4501 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
     4561 gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     4621 tatttcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc
     4681 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cttagcgccc
     4741 gctcctttcg ctttcttccc ttcctttctc gccacgttcg cc
//

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