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RNAi-Ready pSIREN-RetroQ-ZsGreen1

價格:900元

聯系方式:I47-825O-882O

相關技術服務:質粒構建    基因合成    質粒大提

買家導航

載體基本信息

出品公司: Clontech
載體名稱: RNAi-Ready pSIREN-RetroQ-ZsGreen1
質粒類型: 哺乳動物細胞自失活基因沉默逆病毒載體,shRNA
高拷貝/低拷貝: 低拷貝
啟動子: Human U6
克隆方法: 多克隆位點,限制性內切酶
載體大小: 6561 bp
5' 測序引物及序列: U6-F: ATGGACTATCATATGCTTACCGTA (Clontech )
3' 測序引物及序列: --
載體標簽: --
載體抗性: 氨芐
篩選標記: --
備注: 載體在沉默基因 的同時表達ZsGreen1熒光蛋白。
產品目錄號: 632455
穩定性: /
組成型: 誘導型
病毒/非病毒: 逆病毒

載體質粒圖譜和多克隆位點信息

RNAi-Ready pSIREN-RetroQ-ZsGreen1載體圖譜



RNAi-Ready pSIREN-RetroQ-ZsGreen1多克隆位點

RNAi-Ready pSIREN-RetroQ-ZsGreen1載體特征

載體簡介

載體序列

LOCUS     pSIREN-RetroQ-ZsGreen1_linear   6561 bp    DNA   circular                    12-NOV-2009
COMMENT     Created by Clontech Laboratories Inc. 
COMMENT     Cat. No. 632455
COMMENT     This file is created by Vector NTI
            http://www.altiliburda.com/
COMMENT     VNTAUTHORNAME|biofeng.com|
FEATURES             Location/Qualifiers
     misc_feature    4728..5454
                     /label=5'-LTR\(CMV/MSV)
     misc_feature    5484..6293
                     /label=Extended\packaging\signal
     misc_feature    1433..1858
                     /label=3'\MMLV\LTR\(deletion\in\U3)
     CDS             complement(3505..4365)
                     /label=Amp(R)
     misc_feature    2138..2424
                     /label=SV40\Promoter
     promoter        6309..6561
                     /label=Human\U6\promoter
     rep_origin      complement(2744..3343)
                     /label=pUC\origin
     rep_origin      2359..2424
                     /label=SV40\origin
     promoter        6..594
                     /label=CMV\IE\Promoter
     CDS             618..1313
                     /label=ZsGreen1
     misc_feature    6561..1
                     /label=shRNA\Cloning\Site
BASE COUNT     1572 a      1756 c      1626 g      1607 t 
ORIGIN
        1 aattctagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 
       61 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 
      121 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 
      181 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 
      241 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 
      301 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 
      361 attaccatgg tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca 
      421 cggggatttc caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat 
      481 caacgggact ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg 
      541 cgtgtacggt gggaggtcta tataagcaga gctggtttag tgaaccgtca gatccgctag 
      601 cgctaccggt cgccaccatg gcccagtcca agcacggcct gaccaaggag atgaccatga 
      661 agtaccgcat ggagggctgc gtggacggcc acaagttcgt gatcaccggc gagggcatcg 
      721 gctacccctt caagggcaag caggccatca acctgtgcgt ggtggagggc ggccccttgc 
      781 ccttcgccga ggacatcttg tccgccgcct tcatgtacgg caaccgcgtg ttcaccgagt 
      841 acccccagga catcgtcgac tacttcaaga actcctgccc cgccggctac acctgggacc 
      901 gctccttcct gttcgaggac ggcgccgtgt gcatctgcaa cgccgacatc accgtgagcg 
      961 tggaggagaa ctgcatgtac cacgagtcca agttctacgg cgtgaacttc cccgccgacg 
     1021 gccccgtgat gaagaagatg accgacaact gggagccctc ctgcgagaag atcatccccg 
     1081 tgcccaagca gggcatcttg aagggcgacg tgagcatgta cctgctgctg aaggacggtg 
     1141 gccgcttgcg ctgccagttc gacaccgtgt acaaggccaa gtccgtgccc cgcaagatgc 
     1201 ccgactggca cttcatccag cacaagctga cccgcgagga ccgcagcgac gccaagaacc 
     1261 agaagtggca cctgaccgag cacgccatcg cctccggctc cgccttgccc taactcgaga 
     1321 tatcagtggt ccaggctcta gttttgactc aacaatatca ccagctgaag cctatagagt 
     1381 acgagccata gataaaataa aagattttat ttagtctcca gaaaaagggg ggaatgaaag 
     1441 accccacctg taggtttggc aagctagctt aagtaacgcc attttgcaag gcatggaaaa 
     1501 atacataact gagaatagag aagttcagat caaggtcagg aacagatgga acagggtcga 
     1561 ccctagagaa ccatcagatg tttccagggt gccccaagga cctgaaatga ccctgtgcct 
     1621 tatttgaact aaccaatcag ttcgcttctc gcttctgttc gcgcgcttct gctccccgag 
     1681 ctcaataaaa gagcccacaa cccctcactc ggggcgccag tcctccgatt gactgagtcg 
     1741 cccgggtacc cgtgtatcca ataaaccctc ttgcagttgc atccgacttg tggtctcgct 
     1801 gttccttggg agggtctcct ctgagtgatt gactacccgt cagcgggggt ctttcatttg 
     1861 ggggctcgtc cgggatcggg agacccctgc ccagggacca ccgacccacc accgggaggt 
     1921 aagctggctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc 
     1981 cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg 
     2041 cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt agcgatagcg 
     2101 gagtgtagat ccggctgtgg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc 
     2161 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccagg tgtggaaagt 
     2221 ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca 
     2281 tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc gcccattctc 
     2341 cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc tcggcctctg 
     2401 agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc aaaaagctta 
     2461 ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga 
     2521 aataccgcac agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct 
     2581 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc 
     2641 ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg 
     2701 ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg 
     2761 cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg 
     2821 actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac 
     2881 cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca 
     2941 tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt 
     3001 gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc 
     3061 caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag 
     3121 agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac 
     3181 tagaaggaca gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt 
     3241 tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa 
     3301 gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg 
     3361 gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa 
     3421 aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat 
     3481 atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc 
     3541 gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat 
     3601 acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc 
     3661 ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc 
     3721 tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag 
     3781 ttcgccagtt aatagtttgc gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg 
     3841 ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg 
     3901 atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag 
     3961 taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt 
     4021 catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga 
     4081 atagtgtatg cggcgaccga gttgctcttg cccggcgtca acacgggata ataccgcgcc 
     4141 acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc 
     4201 aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc 
     4261 ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc 
     4321 cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca 
     4381 atattattga agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat 
     4441 ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt 
     4501 ctaagaaacc attattatca tgacattaac ctataaaaat aggcgtatca cgaggccctt 
     4561 tcgtcttcaa gaattagctt ggccattgca tacgttgtat ccatatcata atatgtacat 
     4621 ttatattggc tcatgtccaa cattaccgcc atgttgacat tgattattga ctagttatta 
     4681 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata 
     4741 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat 
     4801 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga 
     4861 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc 
     4921 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt 
     4981 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat 
     5041 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag 
     5101 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc 
     5161 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga 
     5221 ggtctatata agcagagctc aataaaagag cccacaaccc ctcactcggc gcgccagtct 
     5281 tccgatagac tgcgtcgccc gggtacccgt attcccaata aagcctcttg ctgtttgcat 
     5341 ccgaatcgtg gtctcgctgt tccttgggag ggtctcctct gagtgattga ctacccacga 
     5401 cgggggtctt tcatttgggg gctcgtccgg gatttggaga cccctgccca gggaccaccg 
     5461 acccaccacc gggaggtaag ctggccagca acttatctgt gtctgtccga ttgtctagtg 
     5521 tctatgtttg atgttatgcg cctgcgtctg tactagttag ctaactagct ctgtatctgg 
     5581 cggacccgtg gtggaactga cgagttctga acacccggcc gcaaccctgg gagacgtccc 
     5641 agggactttg ggggccgttt ttgtggcccg acctgaggaa gggagtcgat gtggaatccg 
     5701 accccgtcag gatatgtggt tctggtagga gacgagaacc taaaacagtt cccgcctccg 
     5761 tctgaatttt tgctttcggt ttggaaccga agccgcgcgt cttgtctgct gcagcgctgc 
     5821 agcatcgttc tgtgttgtct ctgtctgact gtgtttctgt atttgtctga aaattagggc 
     5881 cagactgtta ccactccctt aagtttgacc ttaggtcact ggaaagatgt cgagcggatc 
     5941 gctcacaacc agtcggtaga tgtcaagaag agacgttggg ttaccttctg ctctgcagaa 
     6001 tggccaacct ttaacgtcgg atggccgcga gacggcacct ttaaccgaga cctcatcacc 
     6061 caggttaaga tcaaggtctt ttcacctggc ccgcatggac acccagacca ggtcccctac 
     6121 atcgtgacct gggaagcctt ggcttttgac ccccctccct gggtcaagcc ctttgtacac 
     6181 cctaagcctc cgcctcctct tcctccatcc gccccgtctc tcccccttga acctcctcgt 
     6241 tcgaccccgc ctcgatcctc cctttatcca gccctcactc cttctctagg cgccggaatt 
     6301 gaagatctgg gcaggaagag ggcctatttc ccatgattcc ttcatatttg catatacgat 
     6361 acaaggctgt tagagagata attagaatta atttgactgt aaacacaaag atattagtac 
     6421 aaaatacgtg acgtagaaag taataatttc ttgggtagtt tgcagtttta aaattatgtt 
     6481 ttaaaatgga ctatcatatg cttaccgtaa cttgaaagta tttcgatttc ttggctttat 
     6541 atatcttgtg gaaaggacga g 
//

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