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pHY300PLK

價格:1500元

聯系方式:I47-825O-882O

相關技術服務:質粒構建    基因合成    質粒大提

買家導航

載體基本信息

出品公司: TaKaRa
載體名稱: pHY300PLK
質粒類型: 枯草芽孢桿菌表達載體
高拷貝/低拷貝: --
啟動子: --
克隆方法: 多克隆位點,限制性內切酶
載體大小: 4870 bp
5' 測序引物及序列: --
3' 測序引物及序列: --
載體標簽: --
載體抗性: Ampicillin和Tetracycline
篩選標記: --
備注: --
產品目錄號: --
穩定性: --
組成型: --
病毒/非病毒: --

載體質粒圖譜和多克隆位點信息

pHY300PLK載體圖譜



載體簡介

載體序列

LOCUS       pHY300PLK	4870 bp 	DNA	SYN
DEFINITION  pHY300PLK
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..4870
                     /organism="pHY300PLK"
                     /mol_type="other DNA"
     CDS             complement(100..1386)
                     /label="ORF frame 2"
                     /translation="MVLNVSLPDIANDFNKPPASTNWVNTAFMLTFSIGTAVYGKLSD
                     QLGIKRLLLFGIIINCFGSVIGFVGHSFFSLLIMARFIQGAGAAAFPALVMVVVARYI
                     PKENRGKAFGLIGSIVAMGEGVGPAIGGMIAHYIHWSYLLLIPMITIITVPFLMKLLK
                     KEVRIKGHFDIKGIILMSVGIVFFMLFTTSYSISFLIVSVLSFLIFVKHIRKVTDPFV
                     DPGLGKNIPFMIGVLCGGIIFGTVAGFVSMVPYMMKDVHQLSTAEIGSVIIFPGTMSV
                     IIFGYIGGILVDRRGPLYVLNIGVTFLSVSFLTASFLLETTSWFMTIIIVFVLGGLSF
                     TKTVISTIVSSSLKQQEAGAGMSLLNFTSFLSEGTGIAIVGGLLSIPLLDQRLLPMEV
                     DQSTYLYSNLLLLFSGIIVISWLVTLNVYKHSQRDF*"
     CDS             complement(1683..2687)
                     /label="ORF frame 3"
                     /translation="MGVSFNIMCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVD
                     YFELLHILEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKH
                     GIQSQKVVAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINK
                     NLVGFMRATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLD
                     YDPNVKVQMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHR
                     KRLISYGGLLKEIHKKLNLDDTEEGDLIHTDDDEKADEDGFSIIAMWNWERKNYFIKE
                     *"
     promoter        3219..3247
                     /label="AmpR_promoter"
                     /translation="MGVSFNIMCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVD
                     YFELLHILEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKH
                     GIQSQKVVAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINK
                     NLVGFMRATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLD
                     YDPNVKVQMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHR
                     KRLISYGGLLKEIHKKLNLDDTEEGDLIHTDDDEKADEDGFSIIAMWNWERKNYFIKE
                     *"
     gene            3289..4149
                     /label="Ampicillin"
                     /gene="Ampicillin"
                     /translation="MGVSFNIMCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVD
                     YFELLHILEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKH
                     GIQSQKVVAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQGFRRMMQYKKINK
                     NLVGFMRATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLD
                     YDPNVKVQMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHR
                     KRLISYGGLLKEIHKKLNLDDTEEGDLIHTDDDEKADEDGFSIIAMWNWERKNYFIKE
                     *"
     CDS             3289..4149
                     /label="ORF frame 1"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                     IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                     YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                     DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                     LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                     EIGASLIKHW*"
ORIGIN
    1 aagcttctag agatctgcag gtcgacggat ccccgggaat tcctgttata aaaaaaggat
   61 caattttgaa ctctctccca aagttgatcc cttaacgatt tagaaatccc tttgagaatg
  121 tttatataca ttcaaggtaa ccagccaact aatgacaatg attcctgaaa aaagtaataa
  181 caaattacta tacagataag ttgactgatc aacttccata ggtaacaacc tttgatcaag
  241 taagggtatg gataataaac cacctacaat tgcaatacct gttccctctg ataaaaagct
  301 ggtaaagtta agcaaactca ttccagcacc agcttcctgc tgtttcaagc tacttgaaac
  361 aattgttgat ataactgttt tggtgaacga aagcccacct aaaacaaata cgattataat
  421 tgtcatgaac catgatgttg tttctaaaag aaaggaagca gttaaaaagc taacagaaag
  481 aaatgtaact ccgatgttta acacgtataa aggacctctt ctatcaacaa gtatcccacc
  541 aatgtagccg aaaataatga cactcattgt tccagggaaa ataattacac ttccgatttc
  601 ggcagtactt agctggtgaa catctttcat catataagga accatagaga caaaccctgc
  661 tactgttcca aatataattc ccccacaaag aactccaatc ataaaaggta tatttttccc
  721 taatccggga tcaacaaaag gatctgttac tttcctgata tgttttacaa atatcaggaa
  781 tgacagcacg ctaacgataa gaaaagaaat gctatatgat gttgtaaaca acataaaaaa
  841 tacaatgcct acagacatta gtataattcc tttgatatca aaatgacctt ttatccttac
  901 ttctttcttt aataatttca taagaaacgg aacagtgata attgttatca taggaatgag
  961 tagaagatag gaccaatgaa tataatgggc tatcattcca ccaatcgctg gaccgactcc
 1021 ttctcccatg gctactatcg atccaataag accaaatgct ttacccctat tttcctttgg
 1081 aatatagcgc gcaactacaa ccattacgag tgctggaaat gcagctgcac cagccccttg
 1141 aataaaacga gccataataa gtaaggaaaa gaaagaatgg ccaacaaacc caattaccga
 1201 cccgaaacaa tttattataa ttccaaatag gagtaacctt ttgatgccta attgatcaga
 1261 tagctttcca tatacagctg ttccaatgga aaaggttaac ataaaggctg tgttcaccca
 1321 gtttgtactc gcaggtggtt tattaaaatc atttgcaata tcaggtaatg agacgttcaa
 1381 aaccatttca tttaatacgc taaaaaaaga taaaatgcaa agccaaatta aaatttggtt
 1441 gtgtcgtaaa ttcgattgtg aataggatgt attcacattt caccctccaa taatgagggc
 1501 agacgtagtt tatagggtta atgatacgct tccctctttt aattgaaccc tgttacattc
 1561 attattcatt acacttcata attaattcct cctaaacttg attaaaacat tttaccacat
 1621 ataaactaag ttttaaattc agtatttcat cacttataca acaatatggc ccgtttgttg
 1681 aactactctt taataaaata atttttccgt tcccaattcc acattgcaat aatagaaaat
 1741 ccatcttcat cggctttttc gtcatcatct gtatgaatca aatcgccttc ttctgtgtca
 1801 tcaaggttta attttttatg tatttctttt aacaaaccac cataggagat taacctttta
 1861 cggtgtaaac cttcctccaa atcagacaaa cgtttcaaat tcttttcttc atcatcggtc
 1921 ataaaatccg tatcctttac aggatatttt gcagtttcgt caattgccga ttgtatatcc
 1981 gatttatatt tatttttcgg tcgaatcatt tgaactttta catttggatc atagtctaat
 2041 ttcattgcct ttttccaaaa ttgaatccat tgtttttgat tcacgtagtt ttctgtattc
 2101 ttaaaataag ttggttccac acataccaat acatgcatgt gctgattata agaattatct
 2161 ttattattta ttgtcacttc cgttgcacgc ataaaaccaa caagattttt attaattttt
 2221 ttatattgca tcattcggcg aaatccttga gccatatctg acaaactctt atttaattct
 2281 tcgccatcat aaacattttt aactgttaat gtgagaaaca accaacgaac tgttggcttt
 2341 tgtttaataa cttcagcaac aaccttttgt gactgaatgc catgtttcat tgctctcctc
 2401 cagttgcaca ttggacaaag cctggattta caaaaccaca ctcgatacaa ctttctttcg
 2461 cctgtttcac gattttgttt atactctaat atttcagcac aatcttttac tctttcagcc
 2521 tttttaaatt caagaatatg cagaagttca aagtaatcaa cattagcgat tttcttttct
 2581 ctccatggtc tcacttttcc actttttgtc ttgtccacta aaacccttga tttttcatct
 2641 gaataaatgc tactattagg acacataata ttaaaagaaa cccccatcta tttagttatt
 2701 tgtttggtca cttataactt taacagatgg ggtttttctg tgcaaccaat tttaagggtt
 2761 ttccaatact ttaaaacaca tacataccaa cacttcaacg cacctttcag caactaaaat
 2821 aaaaatgacg ttatttctat atgtatcaag ataagaaaga acaagttcaa aaccatcaaa
 2881 aaaagacacc ttttcaggtg ctttttttat tttataaact cattccctga tctcgacttc
 2941 gttctttttt tacctctcgg ttatgagtta gttcaaattc gttcttttta ggttctaaat
 3001 cgtgtttttc ttggaattgt gctgttttat cctttacctt gtctacaaac cccttaaaaa
 3061 cgtttttaaa ggcttttaag cgtctgtacg ttccttaagg aattattcct tagtgctttc
 3121 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
 3181 gtccgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
 3241 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
 3301 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
 3361 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
 3421 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
 3481 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc
 3541 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
 3601 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
 3661 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
 3721 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
 3781 ccggagctga atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgcagcaatg
 3841 gcaacaacgt tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa
 3901 ttaatagact ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg
 3961 gctggctggt ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt
 4021 gcagcactgg ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt
 4081 caggcaacta tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag
 4141 cattggtaac tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat
 4201 ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct
 4261 taacgtgagt tttcgttcca ctgagcgtca gaccccttaa taagatgatc ttcttgagat
 4321 cgttttggtc tgcgcgtaat ctcttgctct gaaaacgaaa aaaccgcctt gcagggaggt
 4381 ttttcgaagg ttctctgagc taccaactct ttgaaccgag gtaactggct tgcaggagcg
 4441 cagtcaccaa aacttgtcct ttcagtttag ccttaaccgg cgcatgactt caagactaac
 4501 tcctctaaat caattaccag tggctgctgc cagtggtgct tttgcatgtc tttccgggtt
 4561 ggactcaaga cgatagttac cggataaggc gcagcggtcg gactgaacgg ggggttcgtg
 4621 catacagtcc agcttggagc gaactgccta cccggaactg agtgtcaggc gtggaatgag
 4681 acaaacgcgg ccataacagc ggaatgacac cggtaaaccg aaaggcagga acaggagagc
 4741 gcacgaggga gccgccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
 4801 accactgatt tgagcgtcag atttcgtgat gcttgtcagg gggcggagcc tatggaaaaa
 4861 cgctttgccc
//

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